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Recommended Endpoints for Combination Studies Primary metabolic endpoints: Oxygen consumption rate (OCR) via Seahorse XF analysis Extracellular acidification rate (ECAR) Intracellular NAD+/NADH ratio AMPK phosphorylation (Thr172) Lipid accumulation (Oil Red O staining in adipocyte models) Secondary endpoints: Mitochondrial membrane potential (JC-1 assay) ATP production rate Gene expression of PGC-1alpha, TFAM, CPT1 Model Selection In vitro models (3T3-L1 adipocytes, C2C12 myotubes) are appropriate for initial mechanistic work
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